Garlic is a common ingredient in most countries’ cuisines, it also has health properties that encourage its consumption. However, many women in Brazil use garlic as an antimicrobial agent, inserting cloves into the vaginal canal and leaving it overnight. This practice is common among women who want to have their labor outside a hospital, which is impossible with a positive GBS test. A great number of women report that the garlic technique is effective, and the GBS tests turn negative. In Brazil, this method is increasing in popularity, even being recommended by doctors and other health professionals. So, the goal of this study is to verify the true effect of garlic on GBS growth and adhesion to vaginal cells, in addition we want to determine the effect of garlic on Lactobacilli commonly found in the vaginal microbiota, Lactobacillus crispatus and Lactobacillus gasseri. Thus, inferring what is the influence of garlic in the vaginal environment. GBS was obtained from healthy women and the strain used was previously characterized and belongs to the serotype Ia; we obtained commercially available Lactobacilli. GBS and Lactobacilli were incubated together and separately, in the presence or absence of garlic, for 4 hours, in liquid media. After that, a fraction of the content was spread across specific GBS and lactobacilli media, then colonies were counted. Results showed that garlic inhibits GBS growth significantly, while lactobacilli growth remained unaffected. When cultured together, garlic inhibited GBS growth, and did not interfere with L. crispatus or L. gasseri growth. Our results show that garlic can be used to inhibit GBS growth, not affecting other microorganisms. Our future research will explore the effect of garlic on GBS and lactobacilli along with HeLa cells.